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紫外可见分光光度计测亚硝酸盐
时间:2020-03-15 点击次数:2248

紫外可见分光光度计测亚硝酸盐

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YAXIAOSUANYANHANLIANGBIAOZHUNQUXIAN

将作为空白的2.0mL水,2.0mL各浓度亚硝酸钠标准溶液分别与1.0mL水、3.0mL显色剂混合置于试管中,摇匀并在室温下避光保存。1Ml水和3ML显色剂,混匀,室温下避光静置。30Min 后,紫外可见分光光度计参比池里放入水,用紫外可见分光光度计彩票大家乐测量样品540nm处的吸光度。4种亚硝酸钠标准溶液的吸光值对相应的亚硝酸盐根离子含量(200mL溶液中)做曲线,含量以"亚硝酸根离子质量”表示。

XIAOSUANYANHANLIANGBIAOZHUNQUXIAN

在试管中加入0.2ml NADPH溶液,再加入2.0mL作为空白的水或分别加入2.0mL各浓度硝酸钾标准溶液,以及0.8mL硝酸盐还原酶缓冲液,充分混合均匀,在室温下静置1h。再加入3.0mL显色剂混合物,摇匀,室温下避光储存。30Min后,紫外可见分光光度计参比池里放入水用紫外可见分光光度计在波长为540nm处检测其吸光度。用4种硝酸钾标准溶液的吸光值对响应的总硝酸盐、亚硝酸盐的含量(200mL溶液中)做曲线,含量以“亚硝酸根离子质量(mg)表示”

YAXIAOSUANYANHANLIANGDECEDING

比色皿中加入2mL的样品溶液,放入紫外可见分光光度计自动四联样品池。1Ml水和3ML显色剂,混匀,室温下避光静置。30Min 后,紫外可见分光光度计彩票大家乐参比池里放入水,测量样品540nm处的吸光度。

 

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